CBSE Class 12 Biotechnology Syllabus 2023 (PDF) Download CBSE Class 12 Revised Syllabus & Study Material Here

CBSE Class 12 Biology Syllabus 2023 PDF Highlights

Board NameCentral Board of Secondary Education (CBSE)
Level of the ExamNational Level Exam
Post CategoryCBSE Biotechnology Syllabus
Name of the ClassClass 12
Academic Year2023
Syllabus StatusDownload Here

Download CBSE Class 12 Biotechnology Exam Pattern 2023

CLASS XII (2023)


One Paper                                                                                                        Max. Marks 70+30

Time: 3 hrs.

Unit VProtein and Gene Manipulation40
Unit VICell Culture and Genetic Manipulation30

 One paper                                                                                                                  Time: 3 hrs.

Total Marks: 70

Unit-V Protein and Gene Manipulation                                                                       40 Marks

Download the CBSE Class 12 Biotechnology Syllabus 2023 PDF

we have uploaded the latest CBSE Class 12 Biotechnology Syllabus to help the candidates in their exam preparation. knowing the detailed syllabus will help the candidates to get good scores in the examination. Here we have provided the Complete Subject with covered chapters.

Chapter 1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of

recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter 2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)

Chapter 3: Genomics, Proteomics, and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, and Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation                                                         30 Marks

Chapter-1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications

Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter 3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

PRACTICALS                                                                  30 Marks

Note: Every student will be required to do the following experiments during the academic session.

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using internet site NCBI and download a DNAand protein sequence from internet, analyze it and comment on it
  8. Reading of a DNA sequencing gel to arrive at the sequence
  9. Project work

Scheme of Evaluation

Time: 3 Hours                                                                                                        Max. Marks 30

The scheme of evaluation at the end of the session will be as under:

ATwo experiments6+6 (only one computer

based practical)

Practical record04
Viva on Practical04
BProject work
Write up05
Viva on project05


Note:- More emphasis should be given on hands-on work in projects.

Prescribed Books:

  1. A Text Book of Biotechnology – Class XII: Published by CBSE, New Delhi
  2. A Laboratory Manual of Biotechnology – Class XII: Published by CBSE, New Delhi

Assessment Areas (Theory) 2023 Class XII

Biotechnology (045)

Time : 3 hrs.                                                                                     Maximum Marks: 70 Marks



Demonstrate , Knowledge and Understanding

Application of Knowledge / Concepts30%

Analyze, Evaluate and Create





  • Typology of questions: VSA including MCQs, Assertion – Reasoning type questions; SA; LA-I; LA-II; Source-based/ Case-based/ Passage-based/ Integrated assessment
  • An internal choice of approximately 33% would be

Suggestive verbs for various competencies

  • Demonstrate, Knowledge and Understanding

State, name, list, identify, define, suggest, describe, outline, summarize, etc.

·        Application of Knowledge/Concepts

Calculate, illustrate, show, adapt, explain, distinguish, etc.

·         Analyze, Evaluate and Create

Interpret, analyse, compare, contrast, examine, evaluate, discuss, construct, etc.


Click Here to Download the CBSE Class 12 Biotechnology Syllabus 2023 PDF

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